Research Articles
Shotgun Metagenomics: A Comprehensive Guide to Principles, Methods, and Applications in Biomedical Research
This article provides a comprehensive overview of shotgun metagenomic sequencing, a powerful, culture-independent method for analyzing complex microbial communities.
Absolute Quantification in Low-Biomass Samples: A Comprehensive Guide to Methods, Challenges, and Best Practices for Biomedical Research
Accurate absolute quantification of microbial load in low-biomass samples—such as those from skin, respiratory tract, blood, and tumors—is critical for meaningful biological conclusions in biomedical research and drug development.
Correlative AFM-Confocal Microscopy: A Multimodal Framework for Biofilm Nanomechanics and 3D Architecture
This article provides a comprehensive guide for researchers and drug development professionals on integrating Atomic Force Microscopy (AFM) and Confocal Laser Scanning Microscopy (CLSM) for advanced biofilm analysis.
Correcting AFM Artifacts in Heterogeneous Biofilm Samples: A Guide for Reliable Nanoscale Analysis
Atomic Force Microscopy (AFM) provides unparalleled nanoscale resolution for studying biofilm structure, mechanics, and function.
Probing Biofilm Dynamics in Liquid: How Physiological AFM is Revolutionizing Antimicrobial Research
This article explores the transformative role of Atomic Force Microscopy (AFM) performed under physiological, fluid conditions in advancing biofilm research.
AFM Cantilever Functionalization for Biofilm Receptor Studies: Protocols, Applications, and Advanced Techniques
This article provides a comprehensive resource for researchers and drug development professionals on functionalizing Atomic Force Microscopy (AFM) cantilevers to study specific receptor interactions within bacterial biofilms.
Nanomechanical Properties of Extracellular Polymeric Substances (EPS): From Fundamental Characterization to Advanced Drug Delivery Applications
This article provides a comprehensive analysis of the nanomechanical properties of Extracellular Polymeric Substances (EPS), a critical component of microbial biofilms and cellular matrices.
Efflux Pump Inhibitors as Biofilm Disruptors: Efficacy Validation and Strategies to Combat Antimicrobial Resistance
The escalating global crisis of antimicrobial resistance (AMR) is profoundly exacerbated by bacterial biofilms, which confer significant tolerance to conventional antibiotics.
Comparative Mechanisms of Bacterial Persister Formation: From Molecular Pathways to Therapeutic Innovation
This article provides a comprehensive analysis of the mechanisms underlying bacterial persister cell formation across major pathogenic species.
Substrate Control as an Anti-Persister Strategy: Disrupting Metabolic Dormancy to Combat Chronic Infections
This article explores the critical role of substrate availability in the formation and survival of bacterial persister cells, a major cause of chronic and relapsing infections.